RESUMO
As superoxide (*O(2)-) and hydroxyl radical (*OH) have been implicated in pathogenesis of Parkinson's disease, free radical scavenging, antioxidant, and neuroprotective agents have attracted attention as ways to prevent progression. We examined effects of zingerone, an alkaloid extracted from ginger root, on 6-hydroxydopamine (6-OHDA)-induced dopamine (DA) reduction in mouse striatum. Zingerone administration 1 h before and for 6 more days following one intracerebroventricular 6-OHDA injection prevented reductions of striatal DA and its metabolites, and increased serum *O(2)- scavenging activity. Zingerone did not change activities of catalase or glutathione peroxidase in striatum or serum, or *O(2)- scavenging activity in striatum. Treatment with diethyldithiocarbamate, SOD inhibitor, abolished the protective effect of zingerone against 6-OHDA-induced DA reduction. In vitro, zingerone scavenged *O(2)- and *OH and suppressed lipid peroxidation only weakly. Thus, direct antioxidant effects may be a minor component of its putative neuroprotective effect; instead, zingerone acted mainly by increasing systemic superoxide dismutase activity. Effects of zingerone treatment in this model suggest possible value in treatment of Parkinson's disease.
Assuntos
Dopamina/metabolismo , Sequestradores de Radicais Livres/farmacologia , Guaiacol/análogos & derivados , Guaiacol/farmacologia , Neostriado/metabolismo , Oxidopamina/antagonistas & inibidores , Superóxidos/sangue , Simpatolíticos/antagonistas & inibidores , Ácido 3,4-Di-Hidroxifenilacético/metabolismo , Animais , Catalase/metabolismo , Espectroscopia de Ressonância de Spin Eletrônica , Glutationa Peroxidase/metabolismo , Ácido Homovanílico/metabolismo , Radical Hidroxila/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos ICR , Neostriado/efeitos dos fármacos , Proteínas do Tecido Nervoso/metabolismo , Oxidopamina/farmacologia , Simpatolíticos/farmacologiaRESUMO
We investigated the modifications in endogenous antioxidant capacity and oxidative damage in the brain, liver, kidney and testis in mice exposed to bisphenol A (BPA), an environmental endocrine disrupter. Mice were exposed to BPA throughout embryonic/fetal life and during lactation by feeding their pregnant/lactating mothers BPA at 5 or 10 microg per milliliter of drinking water. At the age of four weeks, male mice were sacrificed. Exposure to BPA increased the activity of catalase and glutathione peroxidase in the liver and kidney, respectively. It also increased thiobarbituric acid-reactive substances in the brain, kidney and testis, and decreased the wet weight of the brain, kidney and testis. Our results suggest that exposure to BPA throughout embryonic/fetal life and during infancy induces tissue oxidative stress and peroxidation, ultimately leading to underdevelopment of the brain, kidney and testis.